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Phospho-AMPKα1 (S496)

Cat Number: ABP-0619
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A phospho specific peptide corresponding to residues surrounding S496 of human Ampk Alpha 1.
Reactivity: Hu
Applications:

Western Blot: 1:500 – 1:1000 Immunohistochemistry: 1:50 – 1:100 Immunofluorescence: 1:50 – 1:100

Molecular: 64kDa
Purification: Affinity purification
Synonyms: PRKAA1; AMPK; AMPKa1; protein kinase AMP-activated catalytic subunit alpha 1
Background:

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5′-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATPconsuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed

Form: liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles
ABP-0619-1.jpg

Western blot analysis of extracts of Untreated and treated HUVEC cell lines, using Phospho-Ampka1-S496 Antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA..