Phospho-AMPKα1/2 (T183/172)
| Cat Number: | ABP-0116 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T172 of human AMPKα |
| Reactivity: | Hu, Ms, Rt, Monkey |
| Applications: | WB 1:1,000 IP: 1:50 IHC: 1:50 |
| Molecular Weight: | 64kDa |
| Purification: | Affinity purification |
| Synonyms: | AMPKa1/AMPKa2 |
| Background: | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is thecatalytic subunit of the 5′-prime-AMP-activated protein kinase (AMPK). AMPK is a cellularenergy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activatedby the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of anumber of key metabolic enzymes through phosphorylation. It protects cells fromstresses that cause ATP depletion by switching off ATP-consuming biosyntheticpathways. Alternatively spliced transcript variants encoding distinct isoforms have beenobserved. [provided by RefSeq, Jul 2008] |
| Form: | liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles |
Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKALPHA 1-T183/AMPKALPHA 2-T172 antibody at 1:2000 dilution. C2C12 cells were treated by Oligomycin (0.5uM) for 30 minutes, treated by serum-starvation overnight, treated by Hydrogen Peroxide (2nM) for 15 minutes or treated by AICAR (0.5mM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Immunological Sciences. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat liver using Phospho-AMPKALPHA 1-T183-AMPKALPHA 2-T172 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using Phospho-AMPKALPHA 1-T183-AMPKALPHA 2-T172 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-AMPKALPHA 1-T183-AMPKALPHA 2-T172 antibody) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of C2C12 cells, using 3 ug Phospho-AMPKALPHA 1-T183/AMPKALPHA 2-T172 pAb Western blot was performed from the immunoprecipitate using Phospho-AMPKALPHA 1-T183/AMPKALPHA 2-T172 pAb ) at a dilition of 1:1000. C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes