Phospho-AMPKα1/2 (T183/172)
| Cat Number: | ABP-0883 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T172 of human AMPKα |
| Reactivity: | Hu,Rt |
| Applications: | WB |
| Molecular Weight: | 64kDa |
| Purification: | Affinity purification |
| Synonyms: | AMPKa1/AMPKa2 |
| Background: | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5′-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| Form: | liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles |
Western blot analysis of extracts of various cell lines, using Phospho-AMPKa1-T183/AMPKa2-T172 antibody at 1:1000 dilution.293T and C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.