Top

Phospho-c-Myc (S62)

Cat Number: ABP10261
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Synthesized peptide derived from human c-Myc around the phosphorylation site of S62.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 Immunofluorescence: 1:200-1:1000 ELISA: 1:40000

Molecular: 50 kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen.
Background:

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5′-CAC[GA]TG-3′. Activates the transcription of growth-related genes.Phospho-c-Myc (S62) Polyclonal Antibody detects endogenous levels of c- Myc protein only when phosphorylated at S62.

Form: liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Storage: Store at -20℃. Avoid freeze / thaw cycles.
ABP10261-1.jpg

Western Blot (WB) analysis of 293T, AD293, 22RV1 and HeLa cells, using Anti-Phospho-c-Myc (S62) polyclonal antibody.

ABP10261-2.jpg

Western Blot (WB) analysis of 293 cells using Anti-Phospho-c-Myc (S62) polyclonal antibody

ABP10261-3.jpg

Immunohistochemical analysis of paraffin-embedded Human breast cancer using Anti-Phospho-c-Myc (S62) polyclonal antibody. Antibody was diluted at 1:100 (4°C, overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antibody retrieval. Negative control (right) obtained from antibody was pre-absorbed by immunogen peptide.