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Phospho-Caveolin-1 (Y14)

Cat Number: ABP-0320
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A phospho specific peptide corresponding to residues surrounding Y14 of human CAV1
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:1000 Immunofluorescence: 1:50 – 1:100

Molecular: 20kDa
Purification: Affinity purification
Synonyms: CAV1; BSCL3; CGL3; LCCNS; MSTP085; PPH3; VIP21 ; caveolin-1
Background:

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1

Form: liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
ABP-0320-1.jpg

Western blot analysis of extracts from 3T3 cells using Phospho-CAV1-Y14 antibody Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

ABP-0320-2.jpg

Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-CAV1-Y14 antibody