Phospho-CFL1-S3 pAb
| Cat Number: | ABP-0178 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Reactivity: | Human,Mouse,Rat |
| Applications: | Western Blotting 1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:100-1:200 |
| Molecular Weight: | 19 kDa |
| Purification: | Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser3 of human cofilin. Antibodies are purified by protein A and peptide affinity chromatography |
| Background: | Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).Phospho-Cofilin (Ser3) Antibody detects endogenous levels of cofilin only when phosphory¬lated at Ser3. The antibody may cross-react with phosphory¬lated cofilin 2, the muscle isoform. |
| Form: | liquid |
| Buffer: | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. |
| Storage: | Store at –20°C. Do not aliquot the antibody. |