Phospho-CREB-1 (S121)
| Cat Number: | ABP10276 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser121. AA range:91-140 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1/500 – 1/2000. |
| Molecular Weight: | 43kD |
| Purification: | The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser121. AA range:91-140 Specificity |
| Synonyms: | CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1. |
| Background: | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western blot analysis of lysates from HeLa cells treated with Etoposide 25uM 24h, using CREB (Phospho-Ser121) Antibody. The lane on the right is blocked with the
phospho peptide.
Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen
retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).