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Phospho-CREB1-S133

Cat Number: ABP-0019
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Immunofluorescence analysis of U2OS cell using Phospho-CHEK1-S317 antibody. Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:1000 Immunofluorescence: 1:50 – 1:100

Molecular: 43kDa
Background:

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

Form: liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
ABP-0019-1.jpg

Western blot analysis of extracts of various cell lines, using Phospho-CREB1-S133 antibody

ABP-0019-2.jpg

Immunofluorescence analysis of MCF7 cell using Phospho-CREB1-S133 antibody. Blue: DAPI for nuclear staining.