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Phospho-EGFR (T693)

Cat Number: ABP-0199
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Synthesized peptide derived from human EGFR around the phosphorylation site of T693.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 Immunofluorescence: 1:200-1:1000 ELISA: 1:40000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Background:

Phospho-EGFR (T693) polyclonal antibody detects endogenous levels of EGFR protein only when phosphorylated at T693.

Form: liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at-20°C, and avoid repeat freeze-thaw cycles.
ABP-0199-1.jpg

Western blot analysis of lysates from A431 cells, using EGFR (Phospho-Thr693) Antibody. The lane on the right is blocked with the phospho peptide.

ABP-0199-2.jpg

Western blot analysis of various cells using Phospho-EGFR (T693) Polyclonal Antibody

ABP-0199-3.jpg

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Thr693) Antibody. The picture on the right is blocked with the phospho peptide.

ABP-0199-4.jpg

Immunofluorescence analysis of HUVEC cells, using EGFR (Phospho-Thr693) Antibody. The lane on the right is blocked with the phospho peptide.