Phospho-eIF2α (S51)
| Cat Number: | ABP-0745 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western blotting 1:500-1000 Immunoprecipitation: 1:50-1:100 Immunofluorescence: 1:50-1:200 |
| Molecular Weight: | 38 kDa |
| Purification: | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background: | Phosphorylation of the eukaryotic initiation factor 2 (eIF2) a subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) or heme deficiency (HRI) can phosphorylate the a subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-y and TNF-a induces potent phosphorylation of eIF2a at Ser51 (5,6).: Phospho-eIF2alpha (Ser51) Anti¬body detects endogenous eIF2alpha only when phosphory¬lated at Ser51. The antibody does not recognize elF2alpha phosphorylated at other sites. |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at –20°C. Avoid freeze / thaw cycles. |