Phospho-eNOS-S1177
| Cat Number: | AB-83619 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Reactivity: | Hu, Ms |
| Applications: | WB: 1:500-1:2000 IHC: 1:50-1:200 |
| Molecular Weight: | 133 kDa |
| Purification: | Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser1177 of human eNOS. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. Phospho-eNOS (Ser1177) Antibody detects endogenous levels of eNOS only when phosphorylated at Ser1177. |
| Form: | liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol,pH7.3 |
| Storage: | Store at -20°C, and avoid repeat freeze-thaw cycles. |
Western blot analysis of extracts from bovine aortic endothelial cells (BAECs), untreated or VEGF-treated (50 ng/ml) for the indicated times, using Phospho-eNOS (Ser1177) Antibody (upper) or control eNOS antibody (lower).
tfistern blot analysis of extracts of SKOV3 cells, using Phospho-eNOS-S1177 antibody (AP0515). SKOV3 cells were treated by EGF. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Immunohistochemistry of paraffinembedded mouse pancreas using Phospho-NOS3-S1177 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffinembedded mouse spinal cord using Phospho-NOS3-S1177 antibody at dilution of 1:200 (40x lens).
References
(1) Fulton, D. et al. (2001) J. Pharmacol. Exp. Ther. 299, 818–824. (2) Shaul, P.W. (2002) Annu. Rev. Physiol. 64, 749–774. (3) Chen, Z.P. et al. (1999) FEBS Lett. 443, 285–289. (4) Dimmeler, S. et al. (1999) Nature 399, 601–605. (5) Fulton, D. et al. (1999) Nature 399, 597–601. (6) Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587–16591. (7) Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017–6024.