|Immunogen:||A synthetic phosphorylated peptide around S1177 of human eNOS.|
Western Blot: 1:500 – 1:2000 Immunohistochemistry: 1:50 – 1:200
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
|Buffer:||PBS with 0.02% sodium azide,50% glycerol,pH7.3.|
|Storage:||Store at -20°C, and avoid repeat freeze-thaw cycles.|
Western blot analysis of extracts of SKOV3 cells, using Phospho-eNOS S1177 antibody SKOV3cells were treated by EGF. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-eNOSS1177 antibody at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus. Exposure time: 60s.
Immunohistochemistry of paraffin embedded mouse pancreas using Phospho-NOS3-S1177 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin embedded mouse spinal cord using Phospho-NOS3-S1177 antibody at dilution of 1:200 (40x lens).