Cat Number: AB-83619
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Reactivity: Hu, Ms

WB: 1:500-1:2000 IHC: 1:50-1:200

Molecular: 133 kDa
Purification: Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser1177 of human eNOS. Antibodies are purified by protein A and peptide affinity chromatography.

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. Phospho-eNOS (Ser1177) Antibody detects endogenous levels of eNOS only when phosphorylated at Ser1177.

Form: liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol,pH7.3
Storage: Store at -20°C, and avoid repeat freeze-thaw cycles.

Western blot analysis of extracts from bovine aortic endothelial cells (BAECs), untreated or VEGF-treated (50 ng/ml) for the indicated times, using Phospho-eNOS (Ser1177) Antibody (upper) or control eNOS antibody (lower).


tfistern blot analysis of extracts of SKOV3 cells, using Phospho-eNOS-S1177 antibody (AP0515). SKOV3 cells were treated by EGF. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.


Immunohistochemistry of paraffinembedded mouse pancreas using Phospho-NOS3-S1177 antibody at dilution of 1:200 (40x lens).


Immunohistochemistry of paraffinembedded mouse spinal cord using Phospho-NOS3-S1177 antibody at dilution of 1:200 (40x lens).


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