Phospho-ERK1/ERK2 (p44/p42 MAPK) (T202/Y204)
| Cat Number: | AB-10762-A |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Peptide derived from the protein area including conserved pT-E-pY motif of activated Erk 1,2 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western blotting: 1:2000 Immunohistochemistry: 1:100-500 Immunofluorescence: 1:100-500 Immunoprecipitation: to be determined Immunocytochemistry: 1:100 Dilutions listed only as a recommendation. Optimal dilution should be determined by investigator. Recognizes ERK1 and ERK2 dually phosphorylated at T202/Y204 and T185/Y187, respectively. |
| Form: | liquid |
| Buffer: | Western immunoblotting solutions: - Wash buffer: 1x Tris Buffered Saline (TBS); 0.1% Triton X 100 - Blocking buffer: 1xTBS; 0.1% Triton X-100; 5% BSA (used with the primary antibody) |
| Storage: | The antibody can be stored at 2° - 8° C for 1 month without detectable loss of activity. Antibody can also be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles. |
Detection of ERK1 and ERK2 phosphorylated at T202/Y204 and T185/Y187, respectively. Human HeLa cells were incubated with 200 nM PMA for the indicated times. Total cell lysates in gel sample buffer were resolved by SDS-PAGE, transferred to Immobilon membrane and Immunoblotted with 0.5µg/Ml antiphosphERK1/2. phosphoERK1/2
WESTERN BLOT (WB ) PROTOCOL
Western immunoblotting solutions:
Wash buffer: 1x Tris Buffered Saline (TBS); 0.1% Triton X 100 –
Blocking buffer: 1xTBS; 0.1% Triton X-100; 5% BSA (used with the primary antibody)
For western blots , incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature