Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit Monoclonal Antibody
| Cat Number: | MAB-94754 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | ARC1595 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | A synthetic phosphorylated peptide around T202 & Y204 of human ERK1MK03 ( |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 42kDa/44kDa |
| Purification: | Affinity purification |
| Synonyms: | ERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; p44-ERK1; p44- MAPK |
| Background: | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. |
| Form: | Liquid |
| Buffer: | PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-
T185/Y187 Rabbit mAb at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by
ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL West Pico Plus.
Exposure time: 10s.
Western blot analysis of extracts of C6 cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187
Rabbit mAb at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30
minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL West Pico Plus.
Exposure time: 90s.
Immunohistochemistry of paraffinembedded rat brain using Phospho-ERK1-
T202/Y204 + ERK2-T185/Y187 Rabbit mAb at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffinembedded rat fallopian tube using Phospho-ERK1-T202/Y204 + ERK2- T185/Y187 Rabbit mAb at dilution of 1:100 (40x lens).
Immunofluorescence analysis of Jurkat treated with PMA and Jurkat using Phospho-ERK1-T202/Y204 + ERK2-
T185/Y187 Rabbit mAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.
References:
Product:Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb
Journal:Neural Regen Res
Application:WB
IF:5.135
Species:Rattus norvegicus
PMID:34472493
Title:Identification of key genes involved in axon regeneration and Wallerian degeneration by weighted gene co-expression network analysis
References:
Product:Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb
Journal:Frontiers of Medicine
Application:WB
IF:4.592
Species:Mus musculus
PMID:29556954
Title:Role of chemerin/CMKLR1 in the maintenance of early pregnancy
References:
Product:Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb
Journal:Pediatr Res
Application:WB
IF:3.756
Species:Homo sapiens
PMID:34952935
Title:Possible mechanism of CHI3L1 promoting tonsil lymphocytes proliferation in children with obstructive sleep apnea syndrome
References:
Product: Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb
Journal:Frontiers in pharmacology
Application:IF
IF:5.98
Species:Mus musculus
PMID:35903325
Title:Theaflavin-3,3-Digallate from Black Tea Inhibits Neointima Formation Through Suppression of the PDGFRβ Pathway in Vascular Smooth Muscle Cells – PMC
References:
Product:Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb
Journal:Pharmaceuticals (Basel, Switzerland)
Application:WB
IF:5.21
Species:Homo sapiens
PMID:36678513
Title:Preclinical Studies of Chiauranib Show It Inhibits Transformed Follicular Lymphoma through the VEGFR2/ERK/STAT3 Signaling Pathway – PMC