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Phospho-FAK (Y407)

Cat Number: ABP10416
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Synthesized peptide derived from human FAK around the phosphorylation site of Y407.
Reactivity: Hu, Ms, Rt, Monkey
Applications:

Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 ELISA: 1:5000

Molecular Weight: 125 kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
Synonyms: Focal adhesion kinase 1 FADK 1 Focal adhesion kinase-related nonkinase FRNK Protein phosphatase 1 regulatory subunit 71 PPP1R71 Protein-tyrosine kinase 2 p125FAK pp125FAK
Background:

Phospho-FAK (Y407) Polyclonal Antibody detects endogenous levels of FAK protein only when phosphorylated at Y407. Tissue Specificity: Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.

Form: liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C, and avoid repeat freeze-thaw cycles.
ABP10416-1.jpg

Western Blot (WB) analysis of specific cells using Phospho-FAK (Y407) polyclonal antibody.

ABP10416-2.jpg

Western Blot (WB) analysis of 293T cells using Phospho-FAK (Y407) polyclonal antibody.

ABP10416-3.jpg

Immunohistochemistry analysis of paraffin-embedded human brain, using FAK (Phospho-Tyr407) Antibody. The picture on the right is blocked with the phospho peptide.