Phospho-GSK3β (S9)
| Cat Number: | MAB-94145 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | D85E12 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western blotting 1:1000 Immunohistochemistry 1:100 Immunofluorescence (IF-IC) 1:200 |
| Molecular Weight: | 46 kDa |
| Purification: | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser9 of human GSK-3beta |
| Background: | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphory-lates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3a and Ser9 of GSK-3beta (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).Phospho-GSK-3beta (Ser9) (D85E12) Rabbit mAb detects endogenous levels of GSK-3beta only when phosphorylated at Ser9. This antibody reacts with denatured components of bovine serum, including BSA. |
| Form: | liquid |
| Buffer: | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02% sodium azide. |
| Storage: | at 4-8 °C for short term, at -20°C for longer term. |