Phospho-mTOR (S2448)
| Cat Number: | MAB-94141 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | D9C2 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western blotting 1:1000 Immunohistochemistry 1:100 Immunofluorescence (IF-IC) 1:200 |
| Molecular Weight: | 290 kDa |
| Purification: | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2448 of human mTOR protein. |
| Background: | The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5).When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).Phospho-mTOR (Ser2448) (D9C2) XP® RabbitmAb detects endogenous levels ofmTOR protein only when phosphorylated at Ser2448. |
| Form: | liquid |
| Buffer: | Supplied in PBS containing 50% glycerol ,0.5% BSA and 0.2% sodium Azide |
| Storage: | Store at –20°C. Do not aliquot the antibody. |
Western blot analysis of extracts from serum-starved NIH/3T3 cells, untreated or insulin-treated (150 nM, 5 minutes), alone or in combination with λ-phosphatase, using Phospho-mTOR (Ser2448) (D9C2) Rabbit mAb (upper) or mTOR (7C10) Rabbit mAb
Confocal immunofluorescent analysis of HeLa cells, rapamycin-treated (10 μM for 2 hours, left), insulin-treated (150 nM for 6 minutes, middle) or insulin- and λ-phosphatase-treated (right), using Phospho-mTOR (Ser2448) (D9C2) Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin (red). Blue pseudocolor = DRAQ5® fluorescent DNA dye)
References
(1) Sabers, C.J. et al. (1995) J Biol Chem 270, (2) Brown, E.J. et al. (1994) Nature 369, 756-8. (3) Sabatini, D.M. et al. (1994) Cell 78, 35-43. (4) Gingras, A.C. et al. (2001) Genes Dev 15, 807- (5) Dennis, P.B. et al. (2001) Science 294, 1102-5. (6) Fang, Y. et al. (2001) Science 294, 1942-5. (7) Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31. (8) Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.