Phospho-mTOR (S2448)
| Cat Number: | MAB-94141 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | D9C2 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western blotting 1:1000 Immunohistochemistry 1:100 Immunofluorescence (IF-IC) 1:200 |
| Molecular: | 290 kDa |
| Purification: | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2448 of human mTOR protein. |
| Background: | The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5).When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9). Phospho-mTOR (Ser2448) Rabbit mAb detects endogenous levels of mTOR protein only when phosphorylated at Ser2448. |
| Form: | Liquid |
| Buffer: | Supplied in PBS containing 50% glycerol ,0.5% BSA and 0.2% sodium Azide |
| Storage: | Store at –20°C. Do not aliquot the antibody. |