Phospho-NFκB-p65 (S536)
| Cat Number: | APB-0124 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Ser536 of human NFkB p65 |
| Reactivity: | Hu, Ms, Rt, Monkey |
| Applications: | Western Blotting 1:1000 Immunohistochemistry 1:100-1:300 Immunofluorescence: 1:50-1:200 Immunoprecipitation 1:200- 1:500 |
| Molecular: | "65 kDa " |
| Purification: | Affinity purification |
| Synonyms: | NFKB3; RELA |
| Background: | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappaB moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, NFKB-P65, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, NFKB-P65. Four transcript variants encoding different isoforms have been found for this gene. |
| Form: | liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3, BSA. |
Western blot analysis of extracts from HepG2 cells using Phospho-NFkB p65 (Ser536) antibody.
Western blot (WB) analysis using NFkappaB-p65 (Phosphor Ser536) polyclonal antibody 1.Hela(+) TNFlpha 2. Hela (-)
Immunohistochemical analysis of humanappendix tissue. Anti-Phospho-NFkB-p65 (S536) at 1:200 (4°C, overnight). Antigen retrieval – Sodium Citrate pH6 (>98°C, 20min). Secondary – 1:200 (room temp, 30min).