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Phospho-NFκB-p65 (S536)

Cat Number: APB-0124
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A phospho specific peptide corresponding to residues surrounding Ser536 of human NFkB p65
Reactivity: Hu, Ms, Rt, Monkey
Applications:

Western Blotting 1:1000 Immunohistochemistry 1:100-1:300 Immunofluorescence: 1:50-1:200 Immunoprecipitation 1:200- 1:500

Molecular: "65 kDa "
Purification: Affinity purification
Synonyms: NFKB3; RELA
Background:

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappaB moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, NFKB-P65, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, NFKB-P65. Four transcript variants encoding different isoforms have been found for this gene.

Form: liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3, BSA.

Western blot analysis of extracts from HepG2 cells using Phospho-NFkB p65 (Ser536) antibody.

Western blot (WB) analysis using NFkappaB-p65 (Phosphor Ser536) polyclonal antibody 1.Hela(+) TNFlpha 2. Hela (-)

ABP-0124-3.jpg

Immunohistochemical analysis of humanappendix tissue. Anti-Phospho-NFkB-p65 (S536) at 1:200 (4°C, overnight). Antigen retrieval – Sodium Citrate pH6 (>98°C, 20min). Secondary – 1:200 (room temp, 30min).