Phospho-NOS3 (S1177)
| Cat Number: | ABP-0421 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Reactivity: | Hu, Ms, Rt |
| Applications: | WB 1:1000, IF 1:200-1:1000 |
| Molecular Weight: | 140 kDa |
| Purification: | Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser1177 of human eNOS. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background: | Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation (6,7).Phospho-eNOS (Ser1177) Antibody detects endogenous levels of eNOS only when phosphorylated at Ser1177. |
| Form: | liquid |
| Storage: | Store at -20°C, and avoid repeat freezethaw cycles. |