Phospho-p38 MAPK-T180/Y182 Rabbit Monoclonal Antibody
| Cat Number: | MAB-190319 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | D3F9 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human p38 MAPK around the phosphorylation site of Thr179 and Tyr181. AA range:151-200 |
| Reactivity: | Human,Mouse,Rat |
| Applications: | Western Blot: 1:500-2000 |
| Molecular Weight: | 41 kDa |
| Purification: | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK |
| Background: | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as ERK6 or SAPK3) and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb detects endogenous levels of p38 MAPK only when phosphorylated at Thr180 and Tyr182. This antibody does not cross-react with the phos-phorylated forms of either p42/44 MAPK or SAPK/JNK. |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at –20°C. Avoid freeze / thaw cycles. |
Western blot analysis of extracts from Hela (+UV) , Hela (-UV), K562 (+UV) , K562 (-UV) cells using Phospho-p38 MAPK (Thr180/Tyr182) antibody.
Immunohistochemical analysis of humanappendix tissue. Anti-Phospho-p38 (T180/Y182) at 1:200 (4°C, overnight). Antigen retrieval – Sodium Citrate pH6 (>98°C, 20min). Secondary – 1:200 (room temp, 30min). Negative control – Secondary only
Immunohistochemical analysis of mouse lung tissue. MAB-190319 was diluted at 1:200 (4°C, overnight). Sodium citrate pH6.0 was used for antibody retrieval (>98°C, 20min). Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control plate was secondary antibody only.