Phospho-p38 MAPK-T180/Y182
| Cat Number: | MAB-94381 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | 3D7 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Reactivity: | Hu, Ms, Rt |
| Applications: | WB 1:1000, IHC-p 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000 |
| Molecular Weight: | 43 kDa |
| Purification: | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK. |
| Background: | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as ERK6 or SAPK3) and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).Phospho-p38 MAP Kinase (Thr180/Tyr182) (3D7) Rabbit mAb detects endogenous levels of p38 MAP kinase only when dually phosphorylated at Thr180 and Tyr182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. |
| Form: | liquid |
| Buffer: | Supplied liquid in PBS containing 50% glycerol 0,5% BSA, 0.02% sodium azide. |
| Storage: | Store at –20°C. Avoid freeze / thaw cycles |
immunohistochemical analysis of human-appendix tissue. Anti- Phospho-p38 (T180/Y182) at 1:200 (4°C, overnight). Antigen retrieval – Sodium Citrate pH6 (>98°C, 20min). Secondary – 1:200 (room temp, 30min). Negative control – Secondary only
Immunohistochemical analysis of mouse kidney tissue. was diluted at 1:200 (4°C, overnight). Sodium citrate pH6.0 was used for antibody retrieval (>98°C, 20min). Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control plate was secondary antibody only.
Specificity of Phospho-Erk1/2, Phospho-p38 MAPK and Phospho- SAPK/JNK Rabbit mAb: Western blot analysis of extracts from NIH/3T3 cells treated with PDGF and UV, using Phospho-p38 MAPK Rabbit Phospho-SAPK/JNK Rabbit mAb and Phospho-Erk1/2 Rabbit mAb.
Western blot (WB) analysis of p38 (phospho Thr180/Y182) polyclonal antibody
Immunohistochemical analysis of rat lung tissue. was diluted at 1:200 (4°C, overnight). Sodium citrate pH6.0 was used for antibody retrieval (>98°C, 20min). Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control plate was secondary antibody only.