Phospho-p70 S6k (T229)
| Cat Number: | ABP10859 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Synthesized peptide derived from human p70 S6 kinase alpha around the phosphorylation site of T229. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 Immunofluorescence: 1:200-1:1000 ELISA: 1:5000 |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen. |
| Background: | Phospho-p70 S6 kinase alpha (T229) polyclonal antibody detects endogenous levels of p70 S6 kinase alpha protein only when phosphorylated at T229. |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of lysates from Jurkat cells, using p70 S6 Kinase (Phospho-Thr229) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using p70 S6 Kinase (Phospho-Thr229) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of various cells using Phospho-p70 S6 kinase Alpha (T229) Polyclonal Antibody diluted at 1：500
Western blot analysis of LOVO cells using Phospho-p70 S6 kinase Alpha (T229) Polyclonal Antibody diluted at 1：500
Immunofluorescence analysis of HeLa cell, 1, p70 Kinase Alpha (phospho Thr229) Polyclonal Antibody (Red) was diluted at 1:200 (4°C overnight). Caspase-8 monoclonal Antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).