Phospho-P70 S6K1 (T389) Rabbit Monoclonal Antibody
|Immunogen:||A synthetic phosphorylated peptide around T389 of human P70 S6K|
|Reactivity:||Hu, Ms, Rt|
WB 1:500 – 1:2000
|Synonyms:||PS6K;S6K;S6K-beta-1;S6K1;STK14A;p70 S6KA;p70(S6K)-alpha;p70-S6K;p70-alpha;P70 S6K;RPS6KB1;p70S6KA|
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin)
|Buffer:||PBS with 0.02% sodium azide,50% glycerol,pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Western blot analysis of NIH/3T3, using Phospho-p70 S6K1 (T389) antibody at 1:500 dilution. NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 180s.
Immunofluorescence analysis of C6 cells using Phospho-p70 S6K1 (T389) antibody at dilution of 1:100. C6 cells were treated by Serum-starvation overnight at 37℃.
Immunofluorescence analysis of HeLa cells using Phospho-p70 S6K1 (T389) antibody at dilution of 1:100.
HeLa cells were treated by Serum-starvation overnight at 37℃.