Phospho-PKC (T497)
| Cat Number: | ABP10914 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Synthesized peptide derived from human PKC around the phosphorylation site of T497. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 Immunohistochemistry: 1:100 – 1:300 Immunofluorescence: 1:200 – 1:1000 ELISA: 1:40000 |
| Molecular Weight: | 83kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. |
| Background: | Protein kinase C alpha(PRKCA) Homo sapiens Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western Blot analysis of 293 cells using Phospho-PKC (T497) Polyclonal Antibody diluted at 1：500
Western Blot analysis of 293 cells using Phospho-PKC (T497) Polyclonal Antibody diluted at 1：500
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC-pan (Phospho-Thr497) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of Hela cell. 1,PKC (phospho Thr497) Polyclonal ntibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Immunofluorescence analysis of HepG2 cells, using PKC-pan (Phospho-Thr497) Antibody. The picture on the right is blocked with the phospho peptide.