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Phospho-SMAD1 SER 463/465+SMAD5 Ser463/465+SMAD9 Ser465/467)

Cat Number: ABP-0850
Conjugate: Unconjugated
Size: 100 ug
Clone: Poly
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A phospho specific peptide corresponding to residues surrounding S463 of human Smad1
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000

Molecular: 60 kDa
Purification: Affinity purification
Synonyms: Smad1/Smad5/Smad9
Form: liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
Storage: Store at -20℃. Avoid freeze / thaw cycles.
ABP-0850-1.jpg

Western blot analysis of extracts of HeLa cells, using PhosphoSmad1(Ser463/465)/Smad5(Ser463/465)/Smad 9(Ser465/467) Polyclonal antibody at 1:1000 dilution.HeLa cells were treated by ATP (5 mM) at 30℃ for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus. Exposure time: 60s.