|Reactivity:||Hu, Ms, Rt|
WB 1:1000, IHC-p 1:50-1:100, IF 1:100
|Purification:||Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser727 of human Stat1. Antibodies are purified by protein A and peptide affin-ity chromatography.|
Stat1, while activated in response to a large number of ligands (1), appears to be essential for responsiveness to IFN-a and IFN-g (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding (4). Stat1 has two isoforms, Stat1a (91 kDa) and the splice variant Stat1b (84 kDa). In most cells, both isoforms are activated by IFN-a, but only Stat1a is activated by IFN-g. Stat1 has been found to be inappropriately activated in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is phosphorylated through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway at Ser727 in response to IFN-a and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.Phospho-Stat1 (Ser727) Antibody detects endogenous levels of Stat1a only when phosphorylated at Ser727. This site is deleted in Stat1b. This antibody does not significantly cross-react with the corresponding phosphorylated residues of other Stat proteins.
|Buffer:||Supplied in PBS with 0,02% sodium azide 50% glycerol, pH 7.3|
|Storage:||Store at -20°C, and avoid repeat freeze-thaw cycles.|
Western blot analysis of extracts from 293 cells, using Phospho-STAT1-S727 antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Immunohistochemistry of paraffinembedded human breast carcinoma tissues, using Phospho – STAT1-S727
Immunofluorescence staining of methanol fixed HeLa cells using Phospho Stat1-S727