Phospho-Trk B (Y706) Rabbit Polyclonal Antibody
| Cat Number: | ABP11158 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | Poly |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Trk B around the phosphorylation site of Tyr705. AA range:671-720 |
| Reactivity: | Human;Mouse;Rat |
| Applications: | Western Blot: 1/500 – 1/2000 |
| Molecular Weight: | 92kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | NTRK2; TRKB; BDNF/NT-3 growth factors receptor; GP145-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B |
| Background: | This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western Blot analysis of 293 cells using Phospho-Trk B (Y706) Polyclonal Antibody diluted at 1:1000
Immunofluorescence analysis of Hela cell. 1,Trk B (phospho Tyr706) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488
was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Immunofluorescence analysis of HUVEC cells, using Trk B (Phospho-Tyr705) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Trk B
(Phospho-Tyr705) Antibody