Phospho-TrkB (Y817) NTRK2 Rabbit Monoclonal Antibody
| Cat Number: | MAB-95050 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | EGA-14 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | A synthesized peptide derived from human Phospho-TrkB (Y817). |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500-1:1000 Immunofluorescence:1:50-1:100 |
| Molecular Weight: | ∼140 kDa |
| Purification: | Affinity-chromatography |
| Form: | Liquid |
| Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| Storage: | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Western blot analysis of TrkB using anti-TrkB antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5
hour at RT. The membrane was incubated with rabbit anti-TrkB antigen affinity purified monoclonal antibody at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TrkB at approximately 140 kDa.
Western blot analysis of Phospho-TrkB (Y817) expression in SH-SY5Y cell lysate treated with BDNF.
Immunohistochemical analysis of paraffin-embedded mouse brain cancer, using Phospho-TrkB (Y817) Antibody.
Immunofluorescent analysis using the Antibody at 1:50 dilution.