Top

PIAS1

Cat Number: MAB-94651
Conjugate: Unconjugated
Size: 100 ug
Clone: ST23
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthesized peptide derived from human PIAS1
Reactivity: Hu, Ms
Applications:

WB: 1:500-1:2000

Molecular: 76kDa
Purification: Aff. Pur.
Synonyms: DDXBP1; GBP; GU/RH-II; ZMIZ3
Background:

This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways including the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
MAB-94651-1.jpg

Western blot analysis of extracts of various cell lines,
using PIAS1 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG
(H+L)at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s

MAB-94651-2.jpg

Western blot analysis of extracts of Mouse lung,
using PIAS1 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit
IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 3min.