Phospho NFKB-p50/p105 (Ser337) Rabbit Polyclonal Antibody

Cat Number: ABP11225
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50 around the phosphorylation site of Ser337. AA range:304-353
Reactivity: Human, Mouse, Rat

Western Blot: 1/500 – 1/2000.
Immunohistochemistry: 1/100 – 1/300
Immunofluorescence: 1/200 – 1/1000

Molecular Weight: 105 kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: NFKB1; Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

nuclear factor kappa B subunit 1(NFKB1) Homo sapiens This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by
various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles

Western Blot analysis of 293 cells using Phospho-NFκB-p105/p50 (S337) Polyclonal Antibody diluted at 1:1000


Western blot analysis of lysates from MDA-MB-435 cells,
using NF-kappaB p105/p50 (Phospho-Ser337) Antibody.
The lane on the left is blocked with the phospho peptide.


Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p105/p50
(Phospho-Ser337) Antibody. The picture on the right is blocked with the phospho peptide.