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PKC beta

Cat Number: AB-84147
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-340 of human PKC-beta.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunofluorescence: 1:50 – 1:200

Molecular: 77kDa
Purification: Aff. Pur.
Synonyms: PRKCB; PKCB; PRKCB1; PRKCB2; protein kinase C beta
Background:

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Form: Liquid
Buffer: Store at -20℃. Avoid freeze / thaw cycles.
Storage: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
AB-84147-1.jpg

Western blot analysis of extracts of
various cell lines, using PKC-beta
antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL Basic Kit
Exposure time: 10s.

AB-84147-2.jpg

Immunofluorescence analysis of A431
cells using PKC-beta antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

AB-84147-3.jpg

Immunofluorescence analysis of C6
cells using PKC-beta antibody at
dilution of 1:100. Blue: DAPI for
nuclear staining.

AB-84147-4.jpg

Immunofluorescence analysis of L929
cells using PKC-beta antibody at
dilution of 1:100. Blue: DAPI for
nuclear staining.