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PPAR-γ Rabbit Polyclonal Antibody

Cat Number: AB-E12636
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human PPAR-gamma. AA range:78-127.
Reactivity: Human;Mouse;Rat
Applications:

Western Blot:  1:500-1:2000                                                              Immunofluorescence:   1:50-200
Immunohistochemistry: 1/100 – 1/300.

Molecular Weight: 57kD
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: PPARG; NR1C3; Peroxisome proliferator-activated receptor gamma; PPAR-gamma; Nuclear receptor subfamily 1 group C member 3
Background:

Peroxisome proliferator activated receptor gamma(PPARG) Homo sapiens This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encodedby this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma
has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
AB-E12636-1

Western Blot analysis of HuvEc cells using PPAR-γ Polyclonal Antibody diluted at 1:1000

AB-E12636-2

Immunofluorescence analysis of rat-lung tissue. 1,PPAR-γ Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody
was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture

AB-E12636-3

Immunofluorescence analysis of rat-lung tissue. 1,PPAR-γ Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody
was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

AB-E12636-4

Immunofluorescence analysis of rat-kidney tissue. 1,PPAR-γ Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody
was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

AB-E12636-5

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,PPAR-γ Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

AB-E12636-6

Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,PPAR-γ Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

AB-E12636-7

Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,PPAR-γ Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.