PPAR-γ Rabbit Polyclonal Antibody
Cat Number: | AB-E12636 |
---|---|
Conjugate: | Unconjugated |
Size: | 100 ug |
Clone: | POLY |
Concentration: | 1mg/ml |
Host: | Rabbit |
Isotype: | IgG |
Immunogen: | The antiserum was produced against synthesized peptide derived from human PPAR-gamma. AA range:78-127. |
Reactivity: | Human;Mouse;Rat |
Applications: | Western Blot: 1:500-1:2000 Immunofluorescence: 1:50-200 |
Molecular Weight: | 57kD |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Synonyms: | PPARG; NR1C3; Peroxisome proliferator-activated receptor gamma; PPAR-gamma; Nuclear receptor subfamily 1 group C member 3 |
Background: | Peroxisome proliferator activated receptor gamma(PPARG) Homo sapiens This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encodedby this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma |
Form: | Liquid |
Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western Blot analysis of HuvEc cells using PPAR-γ Polyclonal Antibody diluted at 1:1000
Immunofluorescence analysis of rat-lung tissue. 1,PPAR-γ Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody
was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture
Immunofluorescence analysis of rat-lung tissue. 1,PPAR-γ Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody
was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-kidney tissue. 1,PPAR-γ Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody
was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,PPAR-γ Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,PPAR-γ Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,PPAR-γ Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.