PRDM1
| Cat Number: | AB-84143 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | E. coli-derived human PRDM1/Blimp1 recombinant protein. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western blot: 0.1-0.5μg/ml |
| Purification: | Aff. Pur. |
| Background: | PR domain zinc finger protein 1 also known as BLIMP-1 is a protein that in humans is encoded by the PRDM1 gene. This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. |
| Form: | Liquid |
| Buffer: | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Storage: | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing. |
Western blot analysis of PRDM1/Blimp1 using anti-
PRDM1/Blimp1 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V
(Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat stomach tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse stomach tissue lysates,
Lane 8: mouse lung tissue lysates,
Lane 9: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a
Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked
the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT.
The membrane was incubated with rabbit anti-PRDM1/Blimp1
antigen affinity purified polyclonal antibody at 0.5 μg/mL overnight at 4°C, then washed with
TBS-0.1%Tween 3 times with 5 minutes each and probed with a
goat anti-rabbit IgG-HRP secondary antibody at a dilution of
1:10000 for 1.5 hour at RT. The signal is developed using an
Enhanced Chemiluminescent detection (ECL) kit
with Tanon 5200 system. A specific band was detected
for PRDM1/Blimp1 at approximately 92KD. The expected band
size for PRDM1/Blimp1 is at 92KD.
Western blot analysis of PRDM1/Blimp1 using anti- PRDM1/Blimp1 antibody Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V
(Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human 22RV1 whole cell lysates.
After Electrophoresis, proteins were transferred to a
Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT.The membrane was incubated with rabbit anti-PRDM1/Blimp1 antigen affinity purified polyclonal antibody at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRDM1/Blimp1 at approximately 92KD. The expected band size for PRDM1/Blimp1 is at 92KD.
IHC analysis of PRDM1/Blimp1 using anti-
PRDM1/Blimp1 antibody PRDM1/Blimp1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum.
The tissue section was then incubated with 1μg/ml rabbit anti- PRDM1/Blimp1 Antibody overnight at 4°C.
Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex
(SABC) with DAB as the chromogen.
IHC analysis of PRDM1/Blimp1 using anti-
PRDM1/Blimp1 antibody.
PRDM1/Blimp1 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrievalsolution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRDM1/Blimp1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary
antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of PRDM1/Blimp1 using anti- PRDM1/Blimp1 antibody.
PRDM1/Blimp1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum.
The tissue section was then incubated with 1μg/ml rabbit anti- PRDM1/Blimp1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary
antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.