RAB21
| Cat Number: | AB-84266 |
|---|---|
| Size: | 100ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human RAB21 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 24kDa |
| Purification: | Aff. Pur. |
| Synonyms: | RAB21 |
| Background: | This gene belongs to the Rab family of monomeric GTPases, which are involved in the control of cellular membrane traffic. The encoded protein plays a role in the targeted trafficking of integrins via its association with integrin alpha tails. As a consequence, the encoded protein is involved in the regulation of cell adhesion and migration. Expression of this gene is associated with a poor prognosis for glioma patients. This gene is downregulated by the tumor suppressor miR-200b, and miRNA-200b is itself downregulated in glioma tissues. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using RAB21 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.
Immunofluorescence analysis of C6 cells using RAB21 antibody at dilution of 1:100. Blue: DAPI for
nuclear staining.
Immunofluorescence analysis of U-2 OS cells using RAB21 antibody at dilution of 1:100. Blue:
DAPI for nuclear staining.