RAB5A Rabbit Monoclonal Antibody
| Cat Number: | MAB-94769 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | ARC0297 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 116-215 of human RAB5AA. |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 25kDa |
| Purification: | Affinity purification |
| Synonyms: | RAB5 |
| Background: | Enables GDP binding activity; GTP binding activity; and GTPase activity. Involved in several processes, including amyloid-beta clearance by transcytosis; early endosome to late endosome transport; and regulation of exocytosis. Located in several cellular components, including cytoplasmic side of early endosome membrane; nucleoplasm; and terminal bouton. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using RAB5AA Rabbit mAb at 1：1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico plus.
Exposure time: 30s.
References:
Product:RAB5A Rabbit mAb
Journal:J Cell Sci
Application:WB
IF:5.28
Species:Homo sapiens
PMID:33912962
Title: PDZD8-mediated lipid transfer at contacts between the ER and late endosomes/lysosomes is required for neurite outgrowth
References:
Product:RAB5A Rabbit mAb
Journal:Journal of virology
Application:WB
IF:6.54
Species:Sus scrofa
PMID:36472440
Title:Seneca Valley Virus Enters PK-15 Cells via Caveolae-Mediated Endocytosis and Macropinocytosis Dependent on Low-pH, Dynamin, Rab5, and Rab7