Recombinant Anti-Nestin Mouse Monoclonal Antibody
| Cat Number: | MABS155685 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Clone: | N19 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG1, κ |
| Immunogen: | Recombinant protein corresponding to Mouse Nestin |
| Reactivity: | Species, Mouse, Rat |
| Applications: | WB 1: 500-1: 1500 IHC/IF 1: 750-1: 1500 |
| Molecular: | 208 kDa / 208 kDa |
| Purification: | Affinity purification |
| Synonyms: | NES, Nbla00170, nestin |
| Background: | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Form: | Liquid |
| Buffer: | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
| Storage: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
WB analysis of Nestin. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG , 1: 3000, RT, 1h.
IHC analysis of Nestin. Sample: Mouse brain, 4% PFA 12-24h. Antigen retrieval: Tris-EDTA buffer , 98℃, 20min. Primary antibody: 1: 750, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG , Ready to use, RT, 20min.
IHC analysis of Nestin. Sample: Mouse brain, 4% PFA 12-24h. Antigen retrieval: Tris-EDTA buffer , 98℃, 20min. Primary antibody: 1: 750, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG , Ready to use, RT, 20min.