Recombinant Anti-Phospho-IKB alpha(S32) Rabbit Monoclonal antibody
| Cat Number: | MABS15212 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Clone: | IFK16 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG, κ |
| Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human IκB alpha（S32） |
| Reactivity: | Species, Human, Mouse, Rat |
| Applications: | Western Blot: 1: 500-1: 1000 |
| Purification: | Affinity purification |
| Synonyms: | I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, Ikβ-α, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA |
| Background: | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| Form: | Liquid |
| Buffer: | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
| Storage: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
WB analysis of Phospho-IKB alpha.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 500, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.
WB analysis of Phospho-IKB alpha.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 500, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.
WB analysis of Phospho-IKB alpha.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 500, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.