Recombinant Anti-Vinculin Mouse Monoclonal Antibody
| Cat Number: | MABS150068 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Concentration: | 100ug/100ul |
| Host: | Rabbit |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein corresponding to Human Vinculin |
| Reactivity: | Species, Human, Mouse, Rat |
| Applications: | WB 1: 1000-1: 2000IHC/IF 1: 1000-1: 2000 |
| Molecular: | 124 kDa |
| Purification: | Affinity purification |
| Synonyms: | Metavinculin, Vcl, CMD1W, CMH15, MVCL, MV, VINC |
| Background: | Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. |
| Form: | Liquid |
| Buffer: | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
| Storage: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
WB analysis of Vinculin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Mouse lgG , 1: 5000, RT, 1h.
WB analysis of Vinculin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Mouse lgG , 1: 5000, RT, 1h.
IHC analysis of Vinculin.Sample: Human Renal cancer, 4% PFA 12-24h.Antigen retrieval: Citrate buffer , pressure cooker 2 min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG , Ready to use, RT, 20min.