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RICTOR Monoclonal Antibody

Cat Number: MAB-94699
Conjugate: Unconjugated
Size: 100UG
Clone: MONO
Concentration: 53A2
Host: Rb
Isotype: IgG
Immunogen: rapamycin-insensitive companion of mTOR
Reactivity: Hu, Ms, Rt
Applications:

WB: 1:1000-1:5000; IHC: 1:50-1:2

Molecular Weight: 192 kDa
Purification: Aff. Pur.
Synonyms: AVO3 homolog, hAVO3, KIAA1999, mAVO3, RICTO
Background:

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals.
mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient –
insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin
cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors.
mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a
critical role in AKT1 ‘Ser-473’ phosphorylation, which may facilitate the phosphorylation of the
activation loop of AKT1 on ‘Thr-308’ by PDK1 which is a prerequisite for full activation.
mTORC2 regulates the phosphorylation of SGK1 at ‘Ser-422’. mTORC2 also modulates the
phosphorylation of PRKCA on ‘Ser-657’. Plays an essential role in embryonic growth and
development

Form: Liquid
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Storage: -20° for 12months
MAB-94699-1-1.jpg

Immunohistochemistry of paraffin-embedded
human lung cancer using (RICTOR
antibody) at dilution of 1:100

MAB-94699-2-1.jpg

HEK-293 cells were subjected to SDS PAGE
followed by western blot with
(RICTOR antibody) at dilution of
1:4000