ROCK2 Rabbit Monoclonal Antibody
| Cat Number: | MABN95166 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml. The concentration of this product may be batch-dependent. |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The specific immunogen used to produce this antibody is proprietary information. |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1:2000-1:10000; IF-P/IF-F/IF-ICC 1:200-1:1000; ELISA 1:5000-1:20000 |
| Molecular: | Observed MW: 161 kDa; Calculated MW: 161 kDa |
| Purification: | Affinity Purification |
| Synonyms: | ROCK2; KIAA0619; Rho-associated protein kinase 2; Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; p164ROCK-2 |
| Background: | The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. |
| Form: | Liquid |
| Buffer: | PBS, 50% glycerol, 0.05% Proclin300, 0.05% protective protein |
| Storage: | Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles. |
Western Blot analysis using Hela cell lysate, Proteins were separated by 4-20% SDS-PAGE
Immunohistochemistry analysis of paraffin-embedded Rat brain using ROCK2 Rabbit pAb at dilution of 1:50 (40x lens). High
pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0)
prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon
carcinoma using ROCK2 Rabbit pAb at dilution of 1:50 (40x lens).
High pressure antigen retrieval performed with 0.01M Citrate buffer
(pH 6.0) prior to IHC staining.