RPA-70 Monoclonal Antibody
| Cat Number: | MAB-94700 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100ug |
| Clone: | EPR3472 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPA70/RPA1 (P27694). |
| Reactivity: | Hu, Rt |
| Applications: | WB 1:500 – 1:2000; IP 1:50 – 1:200 |
| Molecular Weight: | 70kDa |
| Purification: | Aff. Pr |
| Synonyms: | HSSB; MST075; REPA1; RF-A; RP-A; RPA70 |
| Background: | As part of the heterotrimeric replication protein A complex (RPA/RP-A, binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. |
| Form: | Liquid |
| Buffer: | PBS with 0.01% thiomersal, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. |
Western blot analysis of extracts of various cell lines, using RPA70/RPA1 Rabbit mAb (A3367) at
1：1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug RPA70/RPA1 antibody
(A3367). Western blot was performed from the immunoprecipitate using RPA70/RPA1 antibody
(A3367) at a dilution of 1:1000.