SHP-1 Rabbit Polyclonal Antibody
| Cat Number: | AB-84762 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human SHP-1. AA range:502-551 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1/500 – 1/2000 |
| Molecular Weight: | 67kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatograp |
| Synonyms: | PTPN6; HCP; PTP1C; Tyrosine-protein phosphatase non-receptor type 6; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; PTP-1C; Protein-tyrosinephosphatase SHP-1; SH-PTP1 |
| Background: | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western Blot analysis of 3T3 cells using SH-PTP1 Polyclonal Antibody
Western blot analysis of lysates from NIH/3T3 cells, using SHP-1 Antibody. The lane on the right is blocked with the
synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4°
overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SHP-1 Antibody.
The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of mouse spleen using SHP1 at dilution of 1:100 (40x lens). Blue: DAPI for
nuclear staining.