SMAD1
| Cat Number: | AB-82128 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Smad1(253-267aa AEISRGDVQAVAYEE), different from the related rat sequence by one amino acid, and different from the related human sequence by two amino acids. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Immunocytochemistry , 1:50 -1:200 |
| Molecular Weight: | 52kDa |
| Purification: | Aff. Pur. |
| Background: | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD) (By similarity). May play a role in the initiation and maintenance of spermatogenesis. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1 (By similarity). May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). |
| Form: | Liquid |
| Buffer: | PBS with 0,02% sodium azide, 50% glycerol pH7.3 |
| Storage: | At -20˚C for one year. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing. |
Western blot analysis of extracts of various cell lines, using SMAD1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST
Anti-Smad1 antibody, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti-Smad1
Immunohistochemistry of paraffin-embedded human kidney using SMAD1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SMAD1 antibody .Blue: DAPI for nuclear staining.