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SOD2

Cat Number: MAB-94601
Size: 100 ug
Clone: S2-F
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthesized peptide derived from human SOD2.
Reactivity: Hu, Rt, Ms
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular: 25kDa
Purification: Aff. Pur.
Synonyms: IPO-B;IPOB;MNSOD;MVCD6;Mn-SOD;SOD2
Background:

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
MAB-94601-1.jpg

Western blot analysis of extracts of various cell lines, using SOD2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 1s.

MAB-94601-2.jpg

Immunohistochemistry of paraffinembedded
rat ovary using SOD2 antibody at dilution of 1:100 (40x lens).

MAB-94601-3.jpg

Immunohistochemistry of paraffinembedded
human esophageal using SOD2 antibody at dilution of 1:100 (40x
lens).

MAB-94601-4.jpg

Immunofluorescence analysis of NIH-3T3
cells using SOD2 antibody.
Blue: DAPI for nuclear staining.

MAB-94601-5.jpg

Review for SOD2 Rabbit Monoclonal Antibody
Experiment Type: Western blot(WB)
Sample NPCs
Description: Western blot analysis of extracts of NPCs cells, using SOD2 Polyclonal Antibody.

References