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SOX10

Cat Number: AB-10868
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic Peptide of human SOX10
Reactivity: Hu,Ms,Rt
Applications:

Western Blot: 1:500 – 1:1000
Immunohistochemistry: 1:50 – 1:50
Immunofluorescence: 1:50 – 1:200

Molecular Weight: 55kDa
Purification: Aff. Pur.
Synonyms: SOX10;DOM;PCWH;WS2E;WS4;WS4C
Background:

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-10868-1.jpg

Western blot analysis of extracts of Rat heart, using SOX10 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 180s.

AB-10868-2.jpg

Immunohistochemistry of paraffin-embedded human brain tissue slide using SOX10 antibody at dilution of 1:100

AB-10868-3.jpg

Immunofluorescence analysis of C6 cells using SOX10 Rabbit pAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.