|Immunogen:||A synthetic Peptide of human SOX10|
Western Blot: 1:500 – 1:1000
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.
|Buffer:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Western blot analysis of extracts of Rat heart, using SOX10 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 180s.
Immunohistochemistry of paraffin-embedded human brain tissue slide using SOX10 antibody at dilution of 1:100
Immunofluorescence analysis of C6 cells using SOX10 Rabbit pAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.