SOX2
| Cat Number: | MAB-90964 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ul |
| Clone: | 10F10 |
| Concentration: | 1mg/ml |
| Host: | Ms |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant fragment of human SOX2 expressed in E. Coli. |
| Reactivity: | Hu |
| Applications: | Western Bloting: 1/250 – 1/1000. |
| Molecular Weight: | Calculated MW: 34kDa Observed MW: ~65kDa |
| Purification: | Aff. Pur. |
| Background: | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Form: | Liquid |
| Buffer: | Ascitic fluid containing 0.03% sodium azide. |
| Storage: | Store at 4°, for long term storage, store at -20°. |
Figure 1: Western blot analysis using SOX2 mAb against HEK293 (1) and SOX2-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis of NTERA-2 cells using SOX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and esophageal cancer tissues (right) using SOX2 mouse mAb with DAB staining