|Immunogen:||Purified recombinant fragment of human SOX2 expressed in E. Coli.|
Western Bloting: 1/250 – 1/1000.
|Molecular Weight:||Calculated MW: 34kDa Observed MW: ~65kDa|
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
|Buffer:||Ascitic fluid containing 0.03% sodium azide.|
|Storage:||Store at 4°, for long term storage, store at -20°.|
Figure 1: Western blot analysis using SOX2 mAb against HEK293 (1) and SOX2-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis of NTERA-2 cells using SOX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and esophageal cancer tissues (right) using SOX2 mouse mAb with DAB staining