Cat Number: MAB-94563
Conjugate: Unconjugated
Size: 100 ug
Clone: S9
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthesized peptide derived from human SOX9.
Reactivity: Hu,Ms,Rt

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular Weight: 70kDa
Purification: Aff. Pur.
Synonyms: CMD1;CMPD1;SRA1;SRXX2;SRXY10;SOX9;SRY-box 9

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Form: Liquid
Buffer: Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Western blot analysis of extracts of
various cell lines, using SOX9
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
Detection: ECL West Pico Plus.
Exposure time: 3min.


Immunohistochemistry of paraffinembedded
rat testis using SOX9
antibody at dilution of
1:100 (40x lens).


Immunohistochemistry of paraffinembedded
mouse testis using SOX9
antibody at dilution of 1:100
(40x lens).


Immunohistochemistry of paraffin embedded
human colon using SOX9
antibody at dilution of 1:100
(40x lens).


Immunofluorescence analysis of HeLa
cells using SOX9 antibody.
Blue: DAPI for nuclear staining.