SOX9
| Cat Number: | MAB-94563 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | S9 |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthesized peptide derived from human SOX9. |
| Reactivity: | Hu,Ms,Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 70kDa |
| Purification: | Aff. Pur. |
| Synonyms: | CMD1;CMPD1;SRA1;SRXX2;SRXY10;SOX9;SRY-box 9 |
| Background: | The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. |
| Form: | Liquid |
| Buffer: | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using SOX9
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 3min.
Immunohistochemistry of paraffinembedded
rat testis using SOX9
antibody at dilution of
1:100 (40x lens).
Immunohistochemistry of paraffinembedded
mouse testis using SOX9
antibody at dilution of 1:100
(40x lens).
Immunohistochemistry of paraffin embedded
human colon using SOX9
antibody at dilution of 1:100
(40x lens).
Immunofluorescence analysis of HeLa
cells using SOX9 antibody.
Blue: DAPI for nuclear staining.